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LABORATORY OF PATHOLOGY ONLINE POLICY MANUAL

February 8, 2005

TO: NIH Staff Researchers and Chemical Waste Generators

FROM: Chief, Waste Management Section, EPB, DS, ORS

Chief, Safety Operations Section, OSHB, DS, ORS

SUBJECT: Proper Management of Chemical Waste in Laboratories

Last year, the NIH received a notice from the Maryland Department of the Environment (MDE) and the US Environmental Protection Agency (EPA) for storage and labeling violations. Safety Officers from the Division of Safety continue to visit laboratories on-campus and discuss proper waste management issues with laboratory personnel. On June 19, 2001 , the Maryland Department of the Environment and EPA performed their regular inspection at NIH laboratories and, unfortunately, found similar types of problems. The goal of this letter is to provide researchers with guidance to comply with state and federal regulations involving waste containers.

The guidelines listed below will help researchers meet MDE and EPA requirements:

    1. All chemical waste containers must be properly labeled with the word >Waste =, the accumulation start date (the date you start collecting waste in the container), chemical constituents with estimated concentrations and quantities, the generator =s location and phone number. Chemical Waste Tags are provided with all three- and five-gallon chemical waste containers. Additional tags for other containers (NSN-7530-00-L07-5985) can be purchased from the Self Service Stores. The information must be attached to the container once you begin accumulating waste.
    2. Have the chemical waste removed on a regular basis, no more than 60 days from the start of the accumulation date. Contact the NIH Chemical Waste Service at 496-4710 to schedule chemical waste pickup.

      3. Carrying chemical waste or waste containers across or down the hall is strictly prohibited. Please keep waste containers in your laboratory.

    3. Secondary containment is required for all waste containers and can be obtained from NIH Chemical Waste Services (496-4710).

    4. Containers should not be overfilled and must remain closed, except during the time when waste is physically poured into the container. Funnels, where used, must be removed and sealed in a closed container when not in use. Covered funnels with screw caps will soon be available to researchers upon request. Call Ms. Swati Damle or Mr. David Mohammadi at 496-7990 if you need a covered funnel.

    5. Do not dispose of any chemicals via sinks or other drains to the sanitary sewer. Disposal of chemicals via the sanitary sewer is not allowed by the NIH Washington Suburban Sanitary Commission permit . Additionally, those items regulated as hazardous waste require proper disposal methods per EPA requirements.
    6. Empty bottles: If researchers are generating small quantities of chemical waste, the waste can be collected in small empty bottles. Deface the original label and complete the information as discussed above in item # 1.

      7. To dispose of empty bottles: rinse the bottles three times, collect the rinsates into a chemical waste container and then dispose of empty bottles in Disposable Labware and Broken Glass boxes. Empty containers of acutely toxic chemicals must be disposed of as chemical waste. (See attachment II for the list of acutely toxic chemicals)

      If you prefer not to rinse the empty bottles, please call Chemical Waste Services (496-7990) to have the empty bottles picked up.

    7. Contaminated agarose gels with ethidium bromide, gel star, acrylamide, etc. should be collected in a box or five gallon pail with a double plastic liner. Attach a Chemical Waste Tag as discussed in item # 1.

      8. DO NOT USE MPW (BURN) BOXES TO COLLECT CONTAMINATED AGAROSE GELS .

    8. For disposing of controlled substances, contact Clinical Center Pharmacy at 6-1914 for human use or Veterinary Resources Pharmacy at 6-2527 for nonhuman use.

    9. Aldehyde waste streams contaminated with the biohazardous material: please see attachment I. If you have any concerns call EPB at 496-7990.

    10. For broken thermometers, call the NIH Fire Department (496-2372) for complete clean up and then call Chemical Waste Services (496-4710) for a pick-up. Do not mix this waste with other waste streams.

    11. Chemically contaminated laboratory trash (gloves, paper towels etc.) must be placed in a plastic bag with attached complete Chemical Waste Tag. Call Chemical Waste Services (496-4710) for a pick-up.

      12. DO NOT USE MPW (BURN) BOXES OR AUTOCLAVE BAGS.

    12. Uninterrupted Power Supply (UPS) battery: call SEIB at 496-7131 to remove the battery from the power supply and then call Chemical Waste Services (496-4710).

Adherence to these guidelines will help to ensure NIH compliance with Federal, State and Local regulations and permits. The Division of Safety will work closely with the NIH community and the EPA, State and Local inspectors to minimize potential penalties.

 


If you have any questions, please call Ms. Swati Damle or Mr. David Mohammadi at 496-7990 or your Occupational Safety and Health Specialist at 496-2346.

 

Thank you for your cooperation.

Mr. William K. Floyd Ms. Polly McCarty

Chief, Waste Management Section Chief, Safety Operations Section

cc:

NIH Staff

Attachment I

THE RECOMMENDED DISPOSAL METHODS

FOR

VARIOUS ALDEHYDE WASTES STREAMS

 

 

Waste Description

 

Treatment and Disposal Methods

 

Unused, outdated or spent (used) aldehyde solution and aldehyde wastes as well as formalin and formalin waste

 

Call the NIH Chemical Waste Service at 496-4710 for pick-up

 

Solid tissues preserved in formalin (biohazardous agents may be present)

 

Add Isoloyzer Aldex (NIH Cat. # 6810-00-L06-0438) crystals to the container according to manufacturers = instructions. Package and dispose of in MPW box according to NIH Waste Calender for MPW. (Add 10% Clorox to disinfect if needed to deactivate the bio hazardous material before adding Isolyzer)

 

Radioactive waste containing aldehyde

 

Do not treat these wastes. Label and date the container according to the procedure as in the NIH Waste Calender for multi hazard/mixed waste and call 6-4451 for pick-up.

 

These methods should not be used for wastes that contain other hazardous chemicals in combination with the aldehydes.

Attachment II

List of Acutely Toxic Chemicals

    1. Acetaldehyde, chloro
    2. Acetamide, N-(aminothioxomethyl)
    3. Acetamide, 2-fluoro
    4. Acetic acid, fluoro-sodium salt
    5. 1-Acetyl-2-thiourea
    6. Acrolein
    7. Aldicarb
    8. Aldicarb sulfone
    9. Aldrin
    10. Allyl alcohol
    11. Aluminum phosphide (R, T)
    12. 5-(Aminomethyl)-3-isoxazolol
    13. 4-Aminopyridine
    14. Ammonium picrate
    15. Ammonium vanadate
    16. Argentate(1-), bis(cyano-C)-, potassium
    17. Arsenic acid
    18. Arsenic trioxide (As 2 O 3)
    19. Arsenic pentoxide (As 2 O 5)
    20. Arsine, diethyl-
    21. Arsonous dichloride, phenyl-
    22. Aziridine
    23. Aziridine, 2-methyl
    24. Barium cyanide
    25. Benzenamine, 4-chloro-
    26. Benzenamine, 4-nitro
    27. Benzene, (chloromethyl)-
    28. 1,2-benzenediol, 4-(1-hydroxy-2-(methylamino)ethyl)-,(R) Aslo known as Epinephrine
    29. Benzeneethanamine, alpha,alpha-dimethyl- Also known as alpha alpha-Dimethylphenethylamine
    30. Benzenethiol
    31. 7-benzofuranol,2,3-dihydro-2.2-dimethyl-,methylcarbamate
    32. Benzoic acid, 2-hydroxy-,compd. with (3aS-cis)-1,2,3,3a,8,8a-hexahydro-1,3a,8-trimethylpyrrolo(2,3-b)indol-5-yl methylcarbamate ester (1:1) Also known as Physostigmine salicylate
    33. 2H-1-benzopyran-20one, 4-hydroxy-3-(3-oxo-1-phenylbutyl)-, and sallts, when present at concentration greater than 0.3% Also known as Warfarin and sallts, when present at concentration greater than 0.3%.
    34. Benzyl chloride
    35. Beryllium powder
    36. Bromoacetone
    37. Brucine
    38. 2-Butanone,3,3-dimethyl-1-(methylthio)-,O-((methyl-amino)carbonyl) oxime Also known as Thiofanox
    39. Calcium cyanide
    40. Carbamic acid, (dibutylamino)-thio)methyl-,2,3-dimethyl-7-benzofuranyl ester Also known as Carbosulfan
    41. Carbamic acid, dimethyl-,1-((dimethylamino)carbonyl)-5-methyl-1H-pyrazol-3-yl ester. Also known as Dimetilan
    42. Carbamic acid, dimethyl-,3-dimethyl-1-(1-methyethyl)-l-1H-pyrazol-5-yl ester. Also known as Isolan
    43. Carbamic acid, methyle-,3-methylphenyl ester. Also known as Matolcarb
    44. Carbofuran
    45. Carbon disufide
    46. Carbonic dichloride
    47. Carbosulfan
    48. p-Chloroaniline
    49. 1(o-Chlorophenyl)thiourea
    50. 3-Chloropropionitrile
    51. Copper cyanide
    52. m-Cumenyl methylcarbamate
    53. Cyanides (soluble cyanide salts) not otherwise specified
    54. Cyanogen
    55. Cyanogen chloride
    56. 2-Cyclohexyl-4,6-dinitrophenol
    57. Dichloromethyl ether
    58. Dichlorophenylarsine
    59. Diethylarsine
    60. Diethyl-p-nitrophenyl phosphate
    61. O,O-Diethyl O-pyrazinyl phosphorothioate
    62. Diisopropylfluorophosphate (DFP)
    63. 1,4,5,8-Dimethanonaphthalene,1,2,3,4,10,10-hexachloro-1,4,4,a,5,8,8a-hexahydro-(1alpha,4alpha,4,beta,5,alpha,8alpha,8beta)- Also known as Aldrin
    64. 1,4,5,8-Dimethanonaphthalene,1,2,3,4,10,10-hexachloro-1,4,4,a,5,8,8a-hexahydro-(1alpha,4alpha,4,beta,5,beta,8beta,8abeta)- Also known as Isodrin
    65. 2,7,:3,6-Dimethaanonaphth(2,3,-b)oxirene,3,4,5,6,9,9-hexachloro-1a,2,2a,3,6,6a,7,7a-octahydro-(1aalpha,2beta,2aalpha,3beta,6beta,6aalpha,7,beta,7aalpha)- Also known as Dieldrin
    66. 2,7,:3,6-Dimethaanonaphth(2,3,-b)oxirene,3,4,5,6,9,9-hexachloro-1a,2,2a,3,6,6a,7,7a-octahydro-(1aalpha,2beta,2abeta,3alpha,6alpha,6abeta,7,beta,7aalpha)- and metabolites Also known as Endrin
    67. Alpa,alpha-Dimethylphenethylamine
    68. Dimetilan
    69. 4,6,Dinitro-o-cresol and salts
    70. 2,4-Dinitrophenol
    71. Dinoseb
    72. Diphosphoramide, octamethyl-
    73. Diphosphoric acid, tetraethyl ester
    74. Disulfoton
    75. Dithiobiuret
    76. 1,3-Dithiolane-2-carboxaldehyde,2,4-dimethyl-,O-((methylamino)-carbnyl)oxime
    77. Endisulfan
    78. Endrine and metabolites
    79. Epinephrine
    80. Ethanedinitrile
    81. Ethanimidothioc acid, -(dimethylamino)-N-(((methylamino)carbonyl)oxy-2-oxo-, methyl ester Also known as Oxamyl
    82. Ethanimidothioc acid, -N-(((dimethylamino)-carbonyl)oxy-, methyl ester Also known as Methomyl
    83. Ethyl cyanide
    84. Ethyleneimine
    85. Fluorine
    86. Fluoroacetamide
    87. Fluoroacetic acid, sodium salt
    88. Formaparanate
    89. Fulminic acid, mercury (2+) salt (R,T)
    90. Heptachlor
    91. Hexaethyl tetraphosphate
    92. Hydrazinecarbothioamide
    93. Hydrazine, methyl-
    94. Hydrocyanic acid or hydrogrn cyanide
    95. Hydrogen phosphide
    96. Isodrin
    97. 3-(2H)-Isoxazolone, 5-(aminomethyl)-
    98. Manganese, bis(dimethylcarbamodithioato-S,S =)- Also known as Manganese dimtheyldithiocarbamate
    99. Mercury, (acetato-O)phenyl-
    100. Mercury fulminate (R,T)
    101. Methanamine, N-methyl-N-nitroso
    102. Methane, isocyanato-
    103. Methane, oxybis(chloro- Also known as Dichloromethyl ether (Bis(chloromethyl)ether)
    104. Methanethiol, trichloro-
    105. Methanimidamide,N,N-dimethyl-N = -(3-((methylamino)-carbonyl)oxy)phenyl)-,monohydrochloride Also known as Formetanate hydrochloride
    106. Methanimidamide,N,N-dimethyl-N = -(2-methyl-4((methylamino)-carbonyl)oxy)phenyl)- Also known as Formetanate
    107. 6,9-Methano-2,4,3-benzodioxathiepin,6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-,3-oxide Also known as Endosulfan
    108. 4,7-Methano-1H-indene,1,4,5,6,7,8,8-heptchloro-3a,4,7,7,7a-tetrahydro- Also known as Heptachlor
    109. Methyl hydrazine
    110. Methyl isocyante
    111. 2-Methylactonitrile
    112. Methyl parathion
    113. Metolcarb
    114. alpha-Naphthylthiourea
    115. Nickel carbonyl
    116. Nickel cyanide
    117. Nicotine, and salts
    118. Nitric oxide (NO)
    119. p-Nitroaniline
    120. Nitrogen oxide (NO 2)
    121. Nitroglycerine
    122. N-Nitrosodimethylamine
    123. N-Nitrosomethylvinylamine
    124. Octamethylpyrophosphoramide
    125. Osmium tetroxide
    126. 7-Oxabicyclo(2.2.1)heptane-2,3-dicarboxylic acid Also known as Endothall
    127. Parathion
    128. Phenol, 2-cyclohexyl-4,6-dinitro-
    129. Phenol, 4-(dimethylamino)-3,5-dimethyl-,methylcarbamate (ester) Also known as Mexacarbate
    130. Phenol, (3,5-dimethyl-4 methylthio)-,methylcarbamate Also known as Methiocarb
    131. Phenol, 2,4-dinitro-
    132. Phenol, 2-methyl-4,6-dinitro-, and salts
    133. Phenol, 3-(1-methyl)-, methyl carbamate
    134. Phenol, 3-methyl-5-(1-methylethyl)-methyl carbamate
    135. Phenol, 2-(1-methylpropyl)-4,6-dinitro-
    136. Phenol, 2,4,6-trinitro-,ammonium salt (R)
    137. Phenylmercury acetate
    138. Phenylthiourea
    139. Phosgene
    140. Phosphine
    141. Phosphoric acid, diethyl 4-nitrophenyl ester
    142. Phosphorodithioic acid, O,O-diethyl S-(2(ethylthio)ethyl)ester Also known as Disulfoton
    143. Phosphorodithioic acid, O,O-diethyl S-((ethylthio)methyl)ester Also known as Phorate
    144. Phosphorodithioic acid, O,O-dimethyl S-(2(methylamino)-2oxoethyl))ester Also known as Dimethoate
    145. Phosphorofluoridic acid, bis-(1-methyl-ethyl)ester
    146. Phosphorothioic acid, O,O-diethyl-O-(4-nitrophenyl)ester
    147. Phosphorothioic acid, O,O-diethyl O-pyrazinyl ester
    148. Phosphorothioic acid, O-(4-((dimethylamino)sulfonyl)phenyl) O,O-dimethyl ester Also known as Famphur
    149. Phosphorothioic acid, O,O-dimethyl O-(4-nitrophenyl) ester Also known as Methyl parathion
    150. Physostigmine
    151. Physistigmine salicylate
    152. Plumbane, tetraethyl-
    153. Potassium cyanide
    154. Potassium silver cyanide
    155. Promecarb
    156. Propanal, 2-methyl-2-(methyl-sulfonyl)-O-((methylamino)carbonyl) oxime Aslo known as Aldicarb sulfone
    157. Propanal,2-methyl-2-(methylthio)-O-((methylamino)carbonyl)oxime Also known as Aldicarb
    158. Propanenitrile
    159. Propanenitrile, 3-chloro-
    160. Propanenitrile,2-hydroxy-2-methyl-
    161. 1,2,3-Propanetiol, trinitrate (R)
    162. 2-Propanone, 1-bromo-
    163. Propargyl alcohol
    164. 2-Propenal
    165. 2-Propen-1-ol
    166. 1,2-Propylenenimine
    167. 2-Propyl-1-ol
    168. 4-Pyridinamine
    169. Pyridine, 3-(1methyl-2-pyrrolidinyl)-,(S)- and salts
    170. Pyrrolo(2,30b)indol-5ol,1,2,3,3a,8,8a-hexahydro-1,3a,-8trimethyl-methylcarbamate (ester), (3aS-cis)- Also known as Physostigmine
    171. Selenious acid, dithallium (1+) salt
    172. Selenourea
    173. Silver cyanide
    174. Sodium azide
    175. Sodium cyanide
    176. Strychnidin-10-one, and salts
    177. Strychnidin-10-one,2,3-dimethoxy-
    178. Strychinn, and salts
    179. Sufuric acid, dithallium (1+) salt
    180. Tetraethyldithiopyrophosphate
    181. Tetraethyl lead
    182. Tetraethyl pyrophosphate
    183. Tetranitromethane (R)
    184. Tetraphosphoric acid, hexaethyl ester
    185. Thallic oxide
    186. Thallium oxide Ti 2O 3
    187. Thallium selenite
    188. Thallium sulfate
    189. Thiodiphosphoric acid, tetraethylester
    190. Thiofanox
    191. Thioimidodicarbonic diamide
    192. Thiophenol
    193. Thiosemicarbazide
    194. Thiourea, (2-chlorophenyl)-
    195. Thiourea, 1-naphthalenyl-
    196. Thiiourea, phenyl-
    197. Tirpate
    198. Toxaphene
    199. Trichoromethanethiol
    200. Vanadic acid, ammonium salt
    201. Vanadium pentoxide, V 2O 5
    202. Vinylamine, N-methyl-N-nitroso-
    203. Zinc, bis(dimethylcarbamodithioato-S,S =)-,
    204. Zinc cyanide
    205. Zinc phosphide when present at concentrations greater than 10%
    206. Ziram

Last Updated 12/28/2009 10:41:46 AM

 
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