LOCUS       P8RWB.TXT    7828 BP DS-DNA   CIRCULAR  SYN       17-MAY-2003

DEFINITION  -

ACCESSION   -

KEYWORDS    -

SOURCE      -

FEATURES             Location/Qualifiers

     misc_feature    complement(>29..2016)

                     /note="2kb EcoR1-Bgl frag with SAR in it"

     promoter        <2062..>2405

                     /note="SV40 promoter"

     misc_feature    complement(>2062..2070)

                     /note="SV40 minor late 19S RNA"

     enhancer        2084..2226

                     /note="SV40 enhancer elements"

     misc_feature    2231..2294

                     /note="SV40 21bp repeats"

     rep_origin      2251..>2405

                     /note="SV40 Ori"

     mRNA            2300..2306

                     /note="late-early transcription startpoints"

     mutation        2320..2320

                     /note="G->A kills SELP ATG"

     mRNA            2340..2346

                     /note="early-early transcription start points"

     misc_feature    2405..2405

                     /note="end homology to SV40"

     promoter        2440..2494

                     /note="EM7 promoter"

     CDS             2514..2936

                     /note="Blasticidin R"

     polyA_signal    2966..>3187

                     /note="SV40 Late PolyA"

     enhancer        <3279..3476

                     /note="EF-1alpha promoter core domain"

     promoter        <3279..>4457

                     /note="EF1a promoter and UTR"

     exon            3477..3509

                     /note="EF-1a exon 1"

     intron          3510..4449

                     /note="EF-1a intron A"

     exon            4449..4457

                     /note="EF-1a exon 2 leader"

     CDS             4554..5228

                     /note="dsRed-Express"

     CDS             <5229..>5243

                     /note="artificial tail"

     mRNA            5287..>5875

                     /note="WPRE"

     polyA_signal    5911..6579

                     /note="hEF1a polyA signal"

     rep_origin      6580..7313

                     /note="MB1 Ori"

     promoter        7334..7380

                     /note="EM7 promoter"

     CDS             7401..7775

                     /note="Zeo"

     terminator      7776..7828

                     /note="terminator (rpmB/G)"

BASE COUNT      2045 A      1780 C      1933 G      2070 T         0 OTHER

ORIGIN      -

        1 CCCTGCATCA AGCTTGGTAC CGAGCTCGGA TCTAAATAAA CTTATAAATT GTGAGAGAAA

       61 TTAATGAATG TCTAAGTTAA TGCAGAAACG GAGAGACATA CTATATTCAT GAACTAAAAG

      121 ACTTAATATT GTGAAGGTAT ACTTTCTTTC CACATAAATT TGTAGTCAAT ATGTTCACCC

      181 CAAAAAAGCT GTTTGTTAAC TTGCCAACCT CATTCTAAAA TGTATATAGA AGCCCAAAAG

      241 ACAATAACAA AAATATTCTT GTAGAACAAA ATGGGAAAGA ATGTTCCACT AAATATCAAG

      301 ATTTAGAGCA AAGCATGAGA TGTGTGGGGA TAGACAGTGA GGCTGATAAA ATAGAGTAGA

      361 GCTCAGAAAC AGACCCATTG ATATATGTAA GTGACCTATG AAAAAAATAT GGCATTTTAC

      421 AATGGGAAAA TGATGATCTT TTTCTTTTTT AGAAAAACAG GGAAATATAT TTATATGTAA

      481 AAAATAAAAG GGAACCCATA TGTCATACCA TACACACAAA AAAATTCCAG TGAATTATAA

      541 GTCTAAATGG AGAAGGCAAA ACTTTAAATC TTTTAGAAAA TAATATAGAA GCATGCCATC

      601 ATGACTTCAG TGTAGAGAAA AATTTCTTAT GACTCAAAGT CCTAACCACA AAGAAAAGAT

      661 TGTTAATTAG ATTGCATGAA TATTAAGACT TATTTTTAAA ATTAAAAAAC CATTAAGAAA

      721 AGTCAGGCCA TAGAATGACA GAAAATATTT GCAACACCCC AGTAAAGAGA ATTGTAATAT

      781 GCAGATTATA AAAAGAAGTC TTACAAATCA GTAAAAAATA AAACTAGACA AAAATTTGAA

      841 CAGATGAAAG AGAAACTCTA AATAATCATT ACACATGAGA AACTCAATCT CAGAAATCAG

      901 AGAACTATCA TTGCATATAC ACTAAATTAG AGAAATATTA AAAGGCTAAG TAACATCTGT

      961 GGCAATATTG ATGGTATATA ACCTTGATAT GATGTGATGA GAACAGTACT TTACCCCATG

     1021 GGCTTCCTCC CCAAACCCTT ACCCCAGTAT AAATCATGAC AAATATACTT TAAAAACCAT

     1081 TACCCTATAT CTAACCAGTA CTCCTCAAAA CTGTCAAGGT CATCAAAAAT AAGAAAAGTC

     1141 TGAGGAACTG TCAAAACTAA GAGGAACCCA AGGAGACATG AGAATTATAT GTAATGTGGC

     1201 ATTCTGAATG AGATCCCAGA ACAGAAAAAG AACAGTAGCT AAAAAACTAA TGAAATATAA

     1261 ATAAAGTTTG AACTTTAGTT TTTTTTAAAA AAGAGTAGCA TTAACACGGC AAAGCCATTT

     1321 TCATATTTTT CTTGAACATT AAGTACAAGT CTATAATTAA AAATTTTTTA AATGTAGTCT

     1381 GGAACATTGC CAGAAACAGA AGTACAGCAG CTATCTGTGC TGTCGCCTAA CTATCCATAG

     1441 CTGATTGGTC TAAAATGAGA TACATCAACG CTCCTCCATG TTTTTTGTTT TCTTTTTAAA

     1501 TGAAAAACTT TATTTTTTAA GAGGAGTTTC AGGTTCATAG CAAAATTGAG AGGAAGGTAC

     1561 ATTCAAGCTG AGGAAGTTTT CCTCTATTCC TAGTTTACTG AGAGATTGCA TCATGAATGG

     1621 GTGTTAAATT TTGTCAAATG CTTTTTCTGT GTCTATCAAT ATGACCATGT GATTTTCTTC

     1681 TTTAACCTGT TGATGGGACA AATTACGTTA ATTGATTTTC AAACGTTGAA CCACCCTTAC

     1741 ATATCTGGAA TAAATTCTAC TTGGTTGTGG TGTATATTTT TTGATACATT CTTGGATTCT

     1801 TTTTGCTAAT ATTTTGTTGA AAATGTTTGT ATCTTTGTTC ATGAGAGATA TTGGTCTGTT

     1861 GTTTTCTTTT CTTGTAATGT CATTTTCTAG TTCCGGTATT AAGGTAATGC TGGCCTAGTT

     1921 GAATGATTTA GGAAGTATTC CCTCTGCTTC TGTCTTCTGA AAGAGATTGT AGAAAGTTGA

     1981 TACAATTTTT TTTTCTTTAA ATATTTGATA GAATTCTGCA GGGCCTGAAA TAACCTCTGA

     2041 AAGAGGAACT TGGTTAGGTA CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA

     2101 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT

     2161 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA

     2221 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC

     2281 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATA CAGAGGCCGA GGCCGCCTCG

     2341 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA

     2401 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT

     2461 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ATCATGAAGA

     2521 CCTTCAACAT CTCTCAGCAG GATCTGGAGC TGGTGGAGGT CGCCACTGAG AAGATCACCA

     2581 TGCTCTATGA GGACAACAAG CACCATGTCG GGGCGGCCAT CAGGACCAAG ACTGGGGAGA

     2641 TCATCTCTGC TGTCCACATT GAGGCCTACA TTGGCAGGGT CACTGTCTGT GCTGAAGCCA

     2701 TTGCCATTGG GTCTGCTGTG AGCAACGGGC AGAAGGACTT TGACACCATT GTGGCTGTCA

     2761 GGCACCCCTA CTCTGATGAG GTGGACAGAT CCATCAGGGT GGTCAGCCCC TGTGGCATGT

     2821 GCAGAGAGCT CATCTCTGAC TATGCTCCTG ACTGCTTTGT GCTCATTGAG ATGAATGGCA

     2881 AGCTGGTCAA AACCACCATT GAGGAACTCA TCCCCCTCAA GTACACCAGG AACTAAACCT

     2941 GAATTCGCTA GAGGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA

     3001 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT

     3061 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT

     3121 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA

     3181 TGTGGTAAAA TCGATAAGGA TCCGGGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC

     3241 CCTTCCCAAC AGTTGCAGGT GGAGAAGAGC ATGCGTGAGG CTCCGGTGCC CGTCAGTGGG

     3301 CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG

     3361 GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC

     3421 CTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCTGTG AACGTTCTTT

     3481 TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG

     3541 GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG

     3601 TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT

     3661 AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG

     3721 TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT

     3781 AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG

     3841 GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG

     3901 CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC

     3961 GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG

     4021 CCCCGCCCTG GGCGGCAAGG CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC

     4081 CGCTTCCCGG CCCTGCTGCA GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG

     4141 CGGGTGAGTC ACCCACACAA AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG

     4201 ACTCCACGGA GTACCGGGCG CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA

     4261 CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG

     4321 TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT

     4381 TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC

     4441 CATTTCAGGT GTCGTGAGAA TTCTGTAGAG ATCCCTCGAC CTCGAGATCC ATTGTGCTGG

     4501 ATCTGCGATC TAAGTAAGCT TCGAATTCTG CAGTCGAGCT ACCGGTCGCC ACCATGGCCT

     4561 CCTCCGAGGA CGTCATCAAG GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCGTGA

     4621 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA

     4681 CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC

     4741 CCCAGTTCCA GTACGGCTCC AAGGTGTACG TGAAGCACCC CGCCGACATC CCCGACTACA

     4801 AGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG

     4861 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CTCCTTCATC TACAAGGTGA

     4921 AGTTCATCGG CGTGAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACTATGGGCT

     4981 GGGAGGCCTC CACCGAGCGC CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGATCCACA

     5041 AGGCCCTGAA GCTGAAGGAC GGCGGCCACT ACCTGGTGGA GTTCAAGTCC ATCTACATGG

     5101 CCAAGAAGCC CGTGCAGCTG CCCGGCTACT ACTACGTGGA CTCCAAGCTG GACATCACCT

     5161 CCCACAACGA GGACTACACC ATCGTGGAGC AGTACGAGCG CGCCGAGGGC CGCCACCACC

     5221 TGTTCCTGAG ATCTCGACAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA TATCAAGCTT

     5281 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT

     5341 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT

     5401 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG

     5461 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC

     5521 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC

     5581 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT

     5641 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG

     5701 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG

     5761 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG

     5821 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA

     5881 TACCGTCGGC CCACTGCTCC CTAAACCTGA GCTAGCATTA TCCCTAATAC CTGCCACCCC

     5941 ACTCTTAATC AGTGGTGGAA GAACGGTCTC AGAACTGTTT GTTTCAATTG GCCATTTAAG

     6001 TTTAGTAGTA AAAGACTGGT TAATGATAAC AATGCATCGT AAAACCTTCA GAAGGAAAGG

     6061 AGAATGTTTT GTGGACCACT TTGGTTTTCT TTTTTGCGTG TGGCAGTTTT AAGTTATTAG

     6121 TTTTTAAAAT CAGTACTTTT TAATGGAAAC AACTTGACCA AAAATTTGTC ACAGAATTTT

     6181 GAGACCCATT AAAAAAGTTA AATGAGAAAC CTGTGTGTTC CTTTGGTCAA CACCGAGACA

     6241 TTTAGGTGAA AGACATCTAA TTCTGGTTTT ACGAATCTGG AAACTTCTTG AAAATGTAAT

     6301 TCTTGAGTTA ACACTTCTGG GTGGAGAATA GGGTTGTTTT CCCCCCACAT AATTGGAAGG

     6361 GGAAGGAATA TCATTTAAAG CTATGGGAGG GTTTCTTTGA TTACAACACT GGAGAGAAAT

     6421 GCAGCATGTT GCTGATTGCC TGTCACTAAA ACAGGCCAAA AACTGAGTCC TTGGGTTGCA

     6481 TAGAAAGCTT CATGTTGCTA AACCAATGTT AAGTGAATCT TTGGAAACAA AATGTTTCCA

     6541 AATTACTGGG ATGTGCATGT TGAAACGTGG GTTAATTAAC TAGCCATGAC CAAAATCCCT

     6601 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT

     6661 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA

     6721 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC

     6781 AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC

     6841 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT

     6901 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG

     6961 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC

     7021 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG

     7081 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG

     7141 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT

     7201 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC

     7261 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTAATTAAA

     7321 TTTTTCAAAA GTAGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA

     7381 CTCACTATAG GAGGGCCATC ATGGCCAAGT TGACCAGTGC TGTCCCAGTG CTCACAGCCA

     7441 GGGATGTGGC TGGAGCTGTT GAGTTCTGGA CTGACAGGTT GGGGTTCTCC AGAGATTTTG

     7501 TGGAGGATGA CTTTGCAGGT GTGGTCAGAG ATGATGTCAC CCTGTTCATC TCAGCAGTCC

     7561 AGGACCAGGT GGTGCCTGAC AACACCCTGG CTTGGGTGTG GGTGAGAGGA CTGGATGAGC

     7621 TGTATGCTGA GTGGAGTGAG GTGGTCTCCA CCAACTTCAG GGATGCCAGT GGCCCTGCCA

     7681 TGACAGAGAT TGGAGAGCAG CCCTGGGGGA GAGAGTTTGC CCTGAGAGAC CCAGCAGGCA

     7741 ACTGTGTGCA CTTTGTGGCA GAGGAGCAGG ACTGAGGATA AGAATTGTAA CAAAAAACCC

     7801 CGCCCCGGCG GGGTTTTTTG TTAATTAA

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