LOCUS       P8FWB.TXT    7917 BP DS-DNA   CIRCULAR  SYN       17-MAY-2003

DEFINITION  -

ACCESSION   -

KEYWORDS    -

SOURCE      -

FEATURES             Location/Qualifiers

     misc_feature    complement(>29..2016)

                     /note="2kb EcoR1-Bgl frag with SAR in it"

     mutation        1407..1407

                     /note="A in standard chrom 9 map, G in mine and M83137"

     misc_feature    complement(>2062..2070)

                     /note="SV40 minor late 19S RNA"

     promoter        <2062..>2405

                     /note="SV40 promoter (ĘSELP)"

     enhancer        2084..2226

                     /note="SV40 enhancer elements"

     misc_feature    2231..2294

                     /note="SV40 21bp repeats"

     rep_origin      2251..>2405

                     /note="SV40 Ori"

     mRNA            2300..2306

                     /note="late-early transcription startpoints"

     mutation        2320..2320

                     /note="G->A kills SELP ATG"

     mRNA            2340..2346

                     /note="early-early transcription start points"

     misc_feature    2405..2405

                     /note="end homology to SV40"

     promoter        2440..2494

                     /note="EM7 promoter"

     CDS             2514..2936

                     /note="Blasticidin R"

     polyA_signal    2966..>3187

                     /note="SV40 Late PolyA"

     enhancer        <3278..3475

                     /note="EF-1alpha promoter core domain"

     promoter        <3278..>4456

                     /note="EF1a promoter and UTR"

     misc_difference 3278..>3469

                     /note="rhesus-derived"

     exon            3476..3508

                     /note="EF-1a exon 1"

     intron          3509..4448

                     /note="EF-1a intron A"

     exon            4448..4456

                     /note="EF-1a exon 2 leader"

     CDS             4573..5292

                     /note="EGFP"

     mRNA            5376..>5964

                     /note="WPRE"

     polyA_signal    6000..6668

                     /note="hEF1a polyA signal"

     rep_origin      6669..7402

                     /note="MB1 Ori"

     promoter        7403..7489

                     /note="EM7 promoter"

     promoter        7423..7469

                     /note="EM7 promoter"

     CDS             7490..7864

                     /note="Zeo"

     terminator      7865..7917

                     /note="terminator (rpmB/G)"

BASE COUNT      2081 A      1815 C      1951 G      2070 T         0 OTHER

ORIGIN      -

        1 CCCTGCATCA AGCTTGGTAC CGAGCTCGGA TCTAAATAAA CTTATAAATT GTGAGAGAAA

       61 TTAATGAATG TCTAAGTTAA TGCAGAAACG GAGAGACATA CTATATTCAT GAACTAAAAG

      121 ACTTAATATT GTGAAGGTAT ACTTTCTTTC CACATAAATT TGTAGTCAAT ATGTTCACCC

      181 CAAAAAAGCT GTTTGTTAAC TTGCCAACCT CATTCTAAAA TGTATATAGA AGCCCAAAAG

      241 ACAATAACAA AAATATTCTT GTAGAACAAA ATGGGAAAGA ATGTTCCACT AAATATCAAG

      301 ATTTAGAGCA AAGCATGAGA TGTGTGGGGA TAGACAGTGA GGCTGATAAA ATAGAGTAGA

      361 GCTCAGAAAC AGACCCATTG ATATATGTAA GTGACCTATG AAAAAAATAT GGCATTTTAC

      421 AATGGGAAAA TGATGATCTT TTTCTTTTTT AGAAAAACAG GGAAATATAT TTATATGTAA

      481 AAAATAAAAG GGAACCCATA TGTCATACCA TACACACAAA AAAATTCCAG TGAATTATAA

      541 GTCTAAATGG AGAAGGCAAA ACTTTAAATC TTTTAGAAAA TAATATAGAA GCATGCCATC

      601 ATGACTTCAG TGTAGAGAAA AATTTCTTAT GACTCAAAGT CCTAACCACA AAGAAAAGAT

      661 TGTTAATTAG ATTGCATGAA TATTAAGACT TATTTTTAAA ATTAAAAAAC CATTAAGAAA

      721 AGTCAGGCCA TAGAATGACA GAAAATATTT GCAACACCCC AGTAAAGAGA ATTGTAATAT

      781 GCAGATTATA AAAAGAAGTC TTACAAATCA GTAAAAAATA AAACTAGACA AAAATTTGAA

      841 CAGATGAAAG AGAAACTCTA AATAATCATT ACACATGAGA AACTCAATCT CAGAAATCAG

      901 AGAACTATCA TTGCATATAC ACTAAATTAG AGAAATATTA AAAGGCTAAG TAACATCTGT

      961 GGCAATATTG ATGGTATATA ACCTTGATAT GATGTGATGA GAACAGTACT TTACCCCATG

     1021 GGCTTCCTCC CCAAACCCTT ACCCCAGTAT AAATCATGAC AAATATACTT TAAAAACCAT

     1081 TACCCTATAT CTAACCAGTA CTCCTCAAAA CTGTCAAGGT CATCAAAAAT AAGAAAAGTC

     1141 TGAGGAACTG TCAAAACTAA GAGGAACCCA AGGAGACATG AGAATTATAT GTAATGTGGC

     1201 ATTCTGAATG AGATCCCAGA ACAGAAAAAG AACAGTAGCT AAAAAACTAA TGAAATATAA

     1261 ATAAAGTTTG AACTTTAGTT TTTTTTAAAA AAGAGTAGCA TTAACACGGC AAAGCCATTT

     1321 TCATATTTTT CTTGAACATT AAGTACAAGT CTATAATTAA AAATTTTTTA AATGTAGTCT

     1381 GGAACATTGC CAGAAACAGA AGTACAGCAG CTATCTGTGC TGTCGCCTAA CTATCCATAG

     1441 CTGATTGGTC TAAAATGAGA TACATCAACG CTCCTCCATG TTTTTTGTTT TCTTTTTAAA

     1501 TGAAAAACTT TATTTTTTAA GAGGAGTTTC AGGTTCATAG CAAAATTGAG AGGAAGGTAC

     1561 ATTCAAGCTG AGGAAGTTTT CCTCTATTCC TAGTTTACTG AGAGATTGCA TCATGAATGG

     1621 GTGTTAAATT TTGTCAAATG CTTTTTCTGT GTCTATCAAT ATGACCATGT GATTTTCTTC

     1681 TTTAACCTGT TGATGGGACA AATTACGTTA ATTGATTTTC AAACGTTGAA CCACCCTTAC

     1741 ATATCTGGAA TAAATTCTAC TTGGTTGTGG TGTATATTTT TTGATACATT CTTGGATTCT

     1801 TTTTGCTAAT ATTTTGTTGA AAATGTTTGT ATCTTTGTTC ATGAGAGATA TTGGTCTGTT

     1861 GTTTTCTTTT CTTGTAATGT CATTTTCTAG TTCCGGTATT AAGGTAATGC TGGCCTAGTT

     1921 GAATGATTTA GGAAGTATTC CCTCTGCTTC TGTCTTCTGA AAGAGATTGT AGAAAGTTGA

     1981 TACAATTTTT TTTTCTTTAA ATATTTGATA GAATTCTGCA GGGCCTGAAA TAACCTCTGA

     2041 AAGAGGAACT TGGTTAGGTA CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA

     2101 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT

     2161 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA

     2221 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC

     2281 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATA CAGAGGCCGA GGCCGCCTCG

     2341 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA

     2401 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT

     2461 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ATCATGAAGA

     2521 CCTTCAACAT CTCTCAGCAG GATCTGGAGC TGGTGGAGGT CGCCACTGAG AAGATCACCA

     2581 TGCTCTATGA GGACAACAAG CACCATGTCG GGGCGGCCAT CAGGACCAAG ACTGGGGAGA

     2641 TCATCTCTGC TGTCCACATT GAGGCCTACA TTGGCAGGGT CACTGTCTGT GCTGAAGCCA

     2701 TTGCCATTGG GTCTGCTGTG AGCAACGGGC AGAAGGACTT TGACACCATT GTGGCTGTCA

     2761 GGCACCCCTA CTCTGATGAG GTGGACAGAT CCATCAGGGT GGTCAGCCCC TGTGGCATGT

     2821 GCAGAGAGCT CATCTCTGAC TATGCTCCTG ACTGCTTTGT GCTCATTGAG ATGAATGGCA

     2881 AGCTGGTCAA AACCACCATT GAGGAACTCA TCCCCCTCAA GTACACCAGG AACTAAACCT

     2941 GAATTCGCTA GAGGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA

     3001 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT

     3061 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT

     3121 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA

     3181 TGTGGTAAAA TCGATAAGGA TCCGGGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC

     3241 CCTTCCCAAC AGTTGCGGTG GAGAAGAGCA TGCGTGAGGC TCCGGTGCCC GTCAGTGGGC

     3301 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG

     3361 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCC

     3421 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCTGTGA ACGTTCTTTT

     3481 TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG

     3541 CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT

     3601 GATTCTTGAT CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA

     3661 AGGAGCCCCT TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT

     3721 GCGAATCTGG TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA

     3781 AAATTTTTGA TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG

     3841 CCAAGATCTG CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC

     3901 GTCCCAGCGC ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG

     3961 GGGGTAGTCT CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC

     4021 CCCGCCCTGG GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC

     4081 GCTTCCCGGC CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC

     4141 GGGTGAGTCA CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA

     4201 CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGAGCT TTTGGAGTAC

     4261 GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT

     4321 GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT

     4381 TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC

     4441 ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG ATCCCTCGAC CTCGAGATCC ATTGTGCTGG

     4501 ATCTGCGATC TAAGTAAGCT TCGAATTCTG CAGTCGACGG TACCGCGGGC CCGGGATCCA

     4561 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG

     4621 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC

     4681 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG

     4741 CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC

     4801 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG

     4861 CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG

     4921 GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC

     4981 ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC

     5041 AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC

     5101 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG

     5161 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC

     5221 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG

     5281 CTGTACAAGT AAAGCGGCCG CTCCACCGCG GTGGCGGCCG CTCTAGAACT AGTGGATCCC

     5341 CCGGGCTGCA GGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT

     5401 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG

     5461 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT

     5521 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG

     5581 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC

     5641 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG

     5701 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT

     5761 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC

     5821 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG

     5881 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA

     5941 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGGCC CACTGCTCCC TAAACCTGAG

     6001 CTAGCATTAT CCCTAATACC TGCCACCCCA CTCTTAATCA GTGGTGGAAG AACGGTCTCA

     6061 GAACTGTTTG TTTCAATTGG CCATTTAAGT TTAGTAGTAA AAGACTGGTT AATGATAACA

     6121 ATGCATCGTA AAACCTTCAG AAGGAAAGGA GAATGTTTTG TGGACCACTT TGGTTTTCTT

     6181 TTTTGCGTGT GGCAGTTTTA AGTTATTAGT TTTTAAAATC AGTACTTTTT AATGGAAACA

     6241 ACTTGACCAA AAATTTGTCA CAGAATTTTG AGACCCATTA AAAAAGTTAA ATGAGAAACC

     6301 TGTGTGTTCC TTTGGTCAAC ACCGAGACAT TTAGGTGAAA GACATCTAAT TCTGGTTTTA

     6361 CGAATCTGGA AACTTCTTGA AAATGTAATT CTTGAGTTAA CACTTCTGGG TGGAGAATAG

     6421 GGTTGTTTTC CCCCCACATA ATTGGAAGGG GAAGGAATAT CATTTAAAGC TATGGGAGGG

     6481 TTTCTTTGAT TACAACACTG GAGAGAAATG CAGCATGTTG CTGATTGCCT GTCACTAAAA

     6541 CAGGCCAAAA ACTGAGTCCT TGGGTTGCAT AGAAAGCTTC ATGTTGCTAA ACCAATGTTA

     6601 AGTGAATCTT TGGAAACAAA ATGTTTCCAA ATTACTGGGA TGTGCATGTT GAAACGTGGG

     6661 TTAATTAACT AGCCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG

     6721 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT

     6781 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC

     6841 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC

     6901 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG

     6961 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT

     7021 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT

     7081 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC

     7141 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA

     7201 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA

     7261 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG

     7321 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT

     7381 GGCCTTTTGC TCACATGTTC TTAATTAAAT TTTTCAAAAG TAGTTGACAA TTAATCATCG

     7441 GCATAGTATA TCGGCATAGT ATAATACGAC TCACTATAGG AGGGCCATCA TGGCCAAGTT

     7501 GACCAGTGCT GTCCCAGTGC TCACAGCCAG GGATGTGGCT GGAGCTGTTG AGTTCTGGAC

     7561 TGACAGGTTG GGGTTCTCCA GAGATTTTGT GGAGGATGAC TTTGCAGGTG TGGTCAGAGA

     7621 TGATGTCACC CTGTTCATCT CAGCAGTCCA GGACCAGGTG GTGCCTGACA ACACCCTGGC

     7681 TTGGGTGTGG GTGAGAGGAC TGGATGAGCT GTATGCTGAG TGGAGTGAGG TGGTCTCCAC

     7741 CAACTTCAGG GATGCCAGTG GCCCTGCCAT GACAGAGATT GGAGAGCAGC CCTGGGGGAG

     7801 AGAGTTTGCC CTGAGAGACC CAGCAGGCAA CTGTGTGCAC TTTGTGGCAG AGGAGCAGGA

     7861 CTGAGGATAA GAATTGTAAC AAAAAACCCC GCCCCGGCGG GGTTTTTTGT TAATTAA

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