PACKAGING

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Papillomaviruses	Paper Name	Reference	Lab Name	Distribute?	Resistance	Notes
BPV1	pSheLL	[1]	pXL∫	AddGene.org	kan	Bicistronic, too large to self-package*

CRPV	pCRPVsheLL	[2]	p¢LLw	yes	amp	Bicistronic, too large to self-package*

HPV1	p1sheLL	unpublished	p1LLw	not yet	amp	HPV1 gives poor L1 yield and HPV1 pseudovirus is poorly infectious

HPV2	p2sheLL	unpublished	p2LLw	yes	amp	This plasmid produces good yields of HPV2 prototype L1/L2 particles that have essentially no detectable infectivity on any tested cell line.
	pRaLw	[3] [4]	pRäLw			This variant (EF362755) HPV2 L1 produces pseudoviruses with detectable (but still extremely poor) infectious titer. Harvest 72h post-transfection
	paL2	[3]	pä∫			HPV2 L2 expression plasmid

HPV5	p5sheLL	[5]	p5LLw	yes	amp	Bicistronic, too large to self-package*. HPV5 has high particle to infectivity ratio on many cell types

HPV6	p6sheLLr †	[6]	p6LLw2	yes	amp	† orignal "p6sheLL" had an unexpected duplication in the backbone due to a cloning error. The duplication was repaired in p6sheLLr. p6sheLL and p6sheLLr give essentially identical titer yields for HPV6 pseudovirus production.

HPV11	p11L1w	[7]	p11Lw	yes, Müller MTA	kan	Titer of HPV11 pseudovirus is very poor. Requires Müller MTA.
	p11L2w	[8]	p11∫w	yes, Müller MTA	kan

HPV16	p16sheLL	[5, 9]	p16LLw	yes, Müller MTA	amp	Replaces pXuLL. Bicistronic, too large to self-package*. Requires Müller MTA.
	p16L1L2	[10, 11]	phüL∫, frankenvirus	yes, Müller MTA	zeo	Synthetic HPV capable of self-packaging. Suitable for infectious amplification in 293TT cells. This plasmid is prone to collapse*

HPV18	p18sheLL	[2, 12]	p18LLw	AddGene.org	amp	Bicistronic, too large to self-package*

HPV27	Senger et al	[3]

HPV31	p31sheLL	[2, 13]	p31LLw	yes, Kanda MTA	amp	Bicistronic, too large to self-package*. Requires Kanda MTA.

HPV35	p35sheLL	[14]	5psh35F	yes, contact Simon Beddows	amp	HPV35 L1 and L2 genes codon optimised for mammalian expression. Requires HPA UK MTA

HPV38	Faust et al	[15]		Faust et al

HPV39	p39sheLL	[14]	39pshC	yes, contact Simon Beddows	amp	HPV35 L1 and L2 genes codon optimised for mammalian expression. Requires HPA UK MTA

HPV45	p45sheLL	[2, 5]	p45LLw	AddGene.org	amp	Bicistronic, too large to self-package*

HPV52	p52sheLL	[16]	p52LLw	yes	amp	Bicistronic, too large to self-package*. Poor titer yield. Requires Kanda MTA.

HPV57	Senger et al	[3]		Senger et al

HPV58	p58sheLL	[13]	p58LLw	yes, Kanda MTA	amp	Bicistronic, too large to self-package*. Requires Kanda MTA.

HPV59	p59sheLL	[14]	59pshB	yes, contact Simon Beddows	amp	HPV35 L1 and L2 genes codon optimised for mammalian expression. Requires HPA UK MTA

MusPV1	pMusheLL	unpublished	pMuLLw	Ask	amp	Mammalian expression plasmid with codon modified L1 and L2 genes of Mus musculus papillomavirus type 1*
	pMusPV	[17, 18], unpub.	pMusPV	Ask	zeo	Synthetic Mus musculus papillomavirus type 1 clone

Polyomaviruses
BKV-I	pCAG-BKV	[19]		Nakanishi et al		VP1/2/3 packaging plasmid for BKV serogroup I (isolate KOM5) reporter vectors
	pBKV-Ia	Unpublished	pIaw	Yes	zeo	Codon modified BKV genotype Ia VP1 expression plasmid

BKV-IV	pwB	[20]	pwB	AddGene.org	zeo	VP1 based on BKV serogroup IV isolate A-66H [21]
	pwB2b	[20]	pwB2b	AddGene.org	zeo	BKV-IV VP1/VP2 dual expression plasmid
	pwB3b	[20]	pwB3b	AddGene.org	zeo	BKV-IV VP1/VP3 dual expression plasmid
	ph2b	[20]	ph2b	AddGene.org	zeo	BKV-IV VP2 expression plasmid
	ph3b	[20]	ph3b	AddGene.org	zeo	BKV-IV VP3 expression plasmid
BKV variants	various	MS in prep	Various	Yes	zeo	Codon modified VP1 ORFs from various BKV genotypes

HPyV6	pHPyV6-607a	[22]	pe1D	AddGene.org	zeo	Reference clone of human polyomavirus 6
	p6VP1	[22]	pe1VP1	AddGene.org	zeo	Human polyomavirus 6 VP1 expression construct

HPyV7	pHPyV7-713a	[22]	pe2S	AddGene.org	zeo	Reference clone of human polyomavirus 7
	p7VP1	[22]	pe2VP1	AddGene.org	zeo	Human polyomavirus 7 VP1 expression construct

HPyV10	p10ww	[23, 24]	p10ww	yes	zeo	Reference clone of human polyomavirus 10 (MWPyV)

MCV	pMCV-R17a	[22]	pMCV-L	AddGene.org	kan	Reference clone of wild-type MCV from healthy skin
	pMCV-R17b	[22]	p17A	yes	zeo	wt MCV clone. Seems to replicate a bit better than R71a [25]
	pMCV-16b	unpublished	p16A	yes	zeo	MCV–R17a chimera with NCRR of Asian isolate MCV-16b
	pwM	[26]	pwM	AddGene.org	zeo	Merkel cell polyomavirus (MCV) VP1 expression construct
	ph2m	[26]	ph2m	AddGene.org	zeo	MCV VP2 expression construct
	pwM2m	[27]	pwM2m	yes	zeo	MCV VP1/VP2 dual expression (VP2 expression relatively weak)
	pMshell	unpublished	pMmw	yes	amp	MCV VP1/VP2 expression, too big to self-package*

MPyV	pwP	[26]	pwP	AddGene.org	zeo	Mouse polyomavirus (MPyV) VP1 expression construct
	ph2p	[26]	ph2p	AddGene.org	zeo	MPyV VP2 expression construct
	ph3p	[26]	ph3p	AddGene.org	zeo	MPyV VP3 expression construct

*Plasmids >10kb can be unstable in E. coli.
Growing bacterial starter cultures at 30ºC and/or using Invitrogen STBL2 or Stratagene XL10-Gold bacteria may decrease the risk of plasmid collapse.