Cryoprotection |
Since December 14, 2009 |
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TIPS AND TRICKS - Cryoprotection
Jim Pflugraph (Rigaku): I always try sugars for everything. There is a cryocrystallography webinar at rigaku.com with embedded videos on how to do this. 50% to 100% saturated sugar (sucrose, glucose, trehalose, sorbitol, et al.) in reservoir buffer is usually what I try. Japan AEA: Cryoprotectant Database Artem Evdokimov: Cryoprotection of Delicate Crystals Introduction I
would like to mention that this is not the easiest approach, and
certainly not
the first that we try on a new crystal (I usually start with 20-25%
ethylene
glycol in crystal mother liquor; this seems to be satisfactory for at
least 50%
of crystals I worked with). However, if you have a few crystals to
spare, and
the simpler things did not pan out, this method is worth trying. |
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