Tips and Tricks in Crystallography Dr.
Xinhua Ji (NCI): Before Making SeMet-labeled Macromolecules,
try quick soak with either halides (Dauter & Dauter, 1999, J
Mol Biol 289:93-101; Dauter et al., 2001, Acta
Crystallogr. D 57:239-249) or metals (Sun &
Radaev, 2002, Acta Crystallogr. D 58:1099-1103; Sun
et al., 2002, Acta Crystallogr. D 58:1092-1098). The
beauty of quick soak is fast. In addition, the binding of metal
atoms can be screened with mass spectrometry (Sun & Hammer,
2000, Acta Crystallogr D 56:161-168). Below are the
main features of both quick-soak approaches. Dr. Zbigniew Dauter
and Dr. Peter Sun are among us and are always ready to help.
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This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov). |