Newsletter 518 (July 15, 2024) NIH X-ray Diffraction SIG

 

Graphical user interface, text

Description automatically generated

 

 

June 2024 PUBLICATIONS BY MEMBERS OF THE GROUP

 

1: Elghondakly A, Jermain MD, Winkler WC, Ferré-D'Amaré AR.

Major-groove sequence-specific RNA recognition by LoaP, a paralog of transcription elongation factor NusG.

Structure. 2024 Jun 28:S0969-2126(24)00219-3. doi: 10.1016/j.str.2024.06.001. Epub ahead of print.

PMID: 38959899.

 

2: Schmidt T, Kubatova N, Clore GM

Deconvoluting Monomer- and Dimer-Specific Distance Distributions between Spin Labels in a Monomer/Dimer Mixture Using T1-Edited DEER EPR Spectroscopy.

J Am Chem Soc. 2024 Jun 18. doi: 10.1021/jacs.4c03916. Epub ahead of print. 

PMID: 38888555.

 

3: Torricella F, Tugarinov V, Clore GM

Effects of Macromolecular Cosolutes on the Kinetics of Huntingtin Aggregation Monitored by NMR Spectroscopy.

J PhysChem Lett. 2024 Jun 20;15(24):6375-6382. doi: 10.1021/acs.jpclett.4c01410. Epub 2024 Jun 10. 

PMID: 38857530.

 

4: Avila YI, Rebolledo LP, Skelly E, de Freitas Saito R, Wei H, Lilley D, Stanley RE, Hou YM, Yang H, Sztuba-Solinska J, Chen SJ, Dokholyan NV, Tan C, Li SK, He X, Zhang X, Miles W, Franco E, Binzel DW, Guo P, Afonin KA. 

Cracking the Code: Enhancing Molecular Tools for Progress in Nanobiotechnology.

ACS Appl Bio Mater. 2024 Jun 17;7(6):3587-3604. doi: 10.1021/acsabm.4c00432. Epub 2024 Jun 4.

PMID: 38833534; PMCID: PMC11190997.

 

For timely listing, please send a heads-up E-mail to the Editor upon publication.

 

 

METHODS – Sample Preparation Tips

 

S. Yadav and K.R. Vinothkumar, “Factors affecting macromolecule orientations in thin films formed in cryo-EM” PMID: 38935342

 

S.E.J. Bowman et al., Preparing research samples for safe arrival at centers and facilities: recipes for successful experiments PMID: 38990054

 

 

METHODS – Integrative Structural Biology

 

M. Bazayeva et al., “A database overview of metal-coordination distances in metalloproteins” PMID: 38682667

 

H. Zheng et al., “PinMyMetal: A hybrid learning system to accurately model metal binding sites in macromolecules” PMID: 38463967.

 

M. Bochtler, “X-rays, electrons, and neutrons as probes of atomic matter” PMID: 38412856.

 

 

METHODS – XFEL, 3D-printed liquid sheet jet technology

 

M. Trebbin, “Thinner than a knife’s edge: 3D-printed liquid sheet jet technology for solution phase XFEL experiments” PMID: 37910141.

 

 

METHODS – Micro-ED, AlphaFold-Assisted

 

J.E. Miller et al., “AlphaFold-assisted structure determination of a bacterial protein of unknown function using X-ray and electron crystallography” PMID: 38451205

 

 

METHODS – Cryo-EM

 

C.L. Lawson et al., “Outcomes of the EMDataResource Cryo-EM Ligand Modeling Challenge” PMID: 38343795

 

B. Greber et al., “High-resolution cryo-EM of the human CDK-activating kinase for structure-based drug design” PMID: 38480681

 

G.J. Kleywegt, “Community recommendations on cryoEM data archiving and validation”  PMID: 38358351

 

M. Kschonsak et al., “Cryo-EM reveals an unprecedented binding site for Nav1.7 inhibitors enabling rational design of potent hybrid inhibitors” PMID: 36975198.

 

 

METHODS – SAXS

 

K. Lytje and J.S. Pederson,Validation of electron-microscopy maps using solution small-angle X-ray scatteringPMID: 38935344.

 

 

METHODS – NMR and Ligand Screening

 

M. Bütikofer et al., Rapid Protein-Ligand Affinity Determination by Photoinduced Hyperpolarized NMRPMID: 38957136.

 

 

READINGS & TUTORIALS – Macromolecular Crystallography

 

K.L. Shelley and E.F. Garman, “Identifying and avoiding radiation damage in macromolecular crystallography” PMID: 38700059

 

G. Khusainov et al., “The time revolution in macromolecular crystallography” PMID: 38616866.

 

J.R. Helliwell et al., “The evolution of raw data archiving and the growth of its importance in crystallography” PMID: 38864497.

 

D.J. Haas, “Fifty years on: why macromolecular cryocrystallography has been so successful” IUCr Newsletter (2023) 31(3).

 

W.A. Hendrickson, “Facing the phase problem” PMID: 37668214.

 

 

READINGS & TUTORIALS – Cryo-EM

 

E. Nogales & J. Mahamid, “Bridging structural and cell biology with cryo-electron microscopy” PMID: 38570716.

 

 

DATABASES – Structural Biology


AlphaFold DBBioCycDisProtExPASyHADHIC-UpNDBPDBPDBe, PDB SARS-CoV-2 ResourcesSARS-CoV-2 Related StructuresRaMP-DBvirusMED